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| Registros recuperados: 24 | |
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| MIYAMOTO, Akio; SHIRASUNA, Koumei; HAYASHI, Ken-Go; KAMADA, Daichi; KAWASHIMA, Chiho; KANEKO, Etsushi; ACOSTA, Tomas J.; MATSUI, Motozumi; 宮本, 明夫; 白砂, 孔明; 川島, 千帆; 松井, 基純. |
| Ultrasonography (US) has been applied to the ovary and the uterus of domestic animals from the late 1980s, and established in 1990s as a practical tool for animal production. US made it possible to detect pregnancy at a very early stage and, most importantly, to observe the real-time dynamics of follicular development and hence the discovery of follicular waves. This has greatly contributed to our understanding of ovarian physiology and helped us to develop several "pin-point" protocols for hormonal treatment. While US may not seem to fit preconceived ideas of a "green" technology, it does not contravene environmental priorities, and it is non-invasive ("ethical") and non-hormonal ("clean"). Using the US technology that is now commercially available at a... |
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Palavras-chave: Color Doppler ultrasound; Blood flow; Ovary; Reproduction; Cow. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2983 |
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| LIU, Jinghui; A. MAREY, Mohamed; KOWSAR, Rasoul; HAMBRUCH, Nina; SHIMIZU, Takashi; HANEDA, Shingo; MATSUI, Motozumi; SASAKI, Motoki; HAYAKAWA, Hiroyuki; PFARRER, Christiane; MIYAMOTO, Akio; 羽田, 真悟; 松井, 基純; 佐々木, 基樹; 宮本, 明夫. |
| We have previously shown that polymorphonuclear neutrophils (PMNs) are present in bovine oviduct fluid under physiological conditions, and that the oviduct provides a microenvironment that protects sperm from phagocytosis by PMNs. Alpha 1-acid glycoprotein (AGP) is a major acute-phase protein produced mainly in the liver that has immunomodulatory functions. AGP mRNA is expressed in extrahepatic organs, such as the lung, kidney, spleen, lymph node, uterus, and ovary. Therefore, in this study, we investigated, 1) the local production of AGP in the bovine oviduct, 2) the effect of AGP on the phagocytic activity of PMNs for sperm and superoxide production and 3) the impact of AGP desialylation on the PMN phagocytosis of sperm. The AGP gene was expressed in... |
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Palavras-chave: Alpha 1-acid glycoprotein (AGP); Bovine; Oviduct; Phagocytosis; Sperm. |
| Ano: 2014 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3946 |
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| ACOSTA, Tomas J.; HAYASHI, Ken-Go; MATSUI, Motozumi; MIYAMOTO, Akio; 松井, 基純; 宮本, 明夫. |
| Increase in the blood supply to individual follicles appears to be associated with follicular growth rates and the ability to become the dominant follicle, while reduced thecal vascularity appears to be closely associated with follicular atresia. Therefore, this study aimed to determine the real-time changes in the vascularity of the follicle wall during the first follicular wave in cycling Holstein cows. Normally cycling and lactating cows (n=5) were examined by transrectal color Doppler ultrasonography (the sensitivity for velocity: > 2 mm/sec) to determine the changes in the vasculature of the follicle wall (presence or absence of blood flow) and the diameter of follicles. A new follicular wave and ovulation were induced by GnRH injection at 48 h... |
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Palavras-chave: Blood flow; Follicular wave; Selection; Deviation; Cow. |
| Ano: 2005 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2979 |
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| KAWASHIMA, Chiho; KIDA, Katsuya; HAYASHI, Ken-Go; AMAYA MONTOYA, Carlos; KANEKO, Etsushi; MATSUNAGA, Nobuyoshi; SHIMIZU, Takashi; MATSUI, Motozumi; MIYAKE, Yoh-Ichi; SCHAMS, Dieter; MIYAMOTO, Akio; 川島, 千帆; 木田, 克弥; 清水, 隆; 松井, 基純; 三宅, 陽一; 宮本, 明夫. |
| The aim of the present study was to investigate the changing profiles of plasma metabolic hormones during the ovarian cycles of beef and dairy cattle. We used 16 non-pregnant, non-lactating Japanese Black beef cattle (6 heifers and 10 cows; parity=2.3 ± 0.8) and 12 multiparous Holstein dairy cows (parity=3.0 ± 0.3). Blood samples for hormonal analysis (growth hormone, GH; insulin-like growth factor-I, IGF-1; insulin; and progesterone, P4) were obtained twice weekly for 40 days before artificial insemination for Japanese Black cattle and from 50 to 100 days postpartum for Holstein cows. Luteal phases were considered normal if the P4 concentrations for at least 3 time points over the course of 7 days remained above 1 ng/ml and at least 2 of the time points... |
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Palavras-chave: Cattle; Insulin-like growth factor-I; Ovarian cycle; Progesterone. |
| Ano: 2007 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2984 |
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| ISHIJIMA, Takako; KOBAYASHI, Yoshiyasu; LEE, Dong-Soo; UETA, Yoshiko Yanagimoto; MATSUI, Motozumi; LEE, Jung-Youn; SUWA, Yoshinori; MIYAHARA, Kazuro; SUZUKI, Hiroshi; 松井, 基純; 宮原, 和郎; 鈴木, 宏志; 古林, 与志安. |
| The cryopreservation of ovarian tissues is a technology with significant potential for the preservation of the genetic resource materials of working dogs, including guide dogs for the blind. However, no attempt has been reported on cryopreservation of the canine ovary. Thus, we evaluated a vitrification method for cryopreservation of canine ovaries and determined the potential functionality of vitrified-warmed canine ovaries by means of transplantation into non-obese diabetic-severe combined immunodeficiency (NOD-SCID) mice. All ovarian tissues cryopreserved by vitrification were morphologically normal in terms of histology. Cryopreserved ovaries were transplanted into the ovarian bursa of the NOD-SCID mice, and the xenografts were recovered from 23 of 23... |
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Palavras-chave: Canine; Ovary transfer; Transplantation; Vitrification; Xenograft. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3023 |
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| TAKAHASHI, Hiroto; HANEDA, Shingo; KAYANO, Mitsunori; MATSUI, Motozumi; 羽田, 真悟; 茅野, 光範; 松井, 基純. |
| Because the establishment of pregnancy begins at the uterine horn ipsilateral to the corpus luteum (ipsi-horn) in cattle, levels of progesterone (P4) and receptor expression in the endometrial tissue, which regulate the intrauterine environment for embryo development, may differ between the ipsi-horn and the uterine horn contralateral to corpus luteum (contra-horn). The aim of the present study was to determine the endometrial tissue P4 concentrations and nuclear progesterone receptor (PGR), progesterone receptor membrane component 1 (PGRMC1) and PGRMC2 mRNA expressions in the cranial and middle parts of the uterine horns during the luteal phase. The results showed higher endometrial tissue P4 concentrations in the cranial part of the ipsi-horn than in... |
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Palavras-chave: Bovine; Progesterone; Receptor; Uterus. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4364 |
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| KAWASHIMA, Chiho; KIDA, Katsuya; MATSUHASHI, Miki; MATSUI, Motozumi; SHIMIZU, Takashi; MATSUNAGA, Nobuyoshi; ISHII, Mitsuo; MIYAKE, Yoh-Ichi; MIYAMOTO, Akio; 川島, 千帆; 木田, 克弥; 松井, 基純; 清水, 隆; 石井, 三都夫; 三宅, 陽一; 宮本, 明夫; 松長, 延吉. |
| The aim of our study was to investigate the effects of suckling on reproductive performance and metabolic status of obese (mean body condition score of more than 4.0 on a scale of 1-5) maternal Japanese Black cows during early postpartum period. We used 7 postpartum Japanese Black cattle. Four cows were suckled ad libitum (suckled) until completion of their first artificial insemination (AI), while 3 cows were not suckled at all because they were separated from their calves immediately after parturition (non-suckled). Body weight and plasma concentrations of metabolites and hormones were measured from wk 1 to 9 postpartum. Ovarian activity was detected using plasma progesterone concentration, and all cows received their first AI after application of the... |
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Palavras-chave: Japanese Black cattle; Metabolic status; Obesity; Reproductive performance; Suckling. |
| Ano: 2008 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2991 |
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| HAYASHI, Ken-Go; MATSUI, Motozumi; ACOSTA, Tomas J.; KIDA, Katsuya; MIYAMOTO, Akio; 松井, 基純; 木田, 克弥; 宮本, 明夫. |
| Luteinizing hormone (LH) surge and follicle rupture act as trigger to start corpus luteum (CL) formation. Thus, we aimed to investigate whether a dominant follicle that has not been exposed to an LH surge can become a functional CL. For this purpose, follicular fluid from the dominant follicles (DF) of cows was aspirated before or after a GnRH-induced LH surge, and subsequent CL formation was observed. Holstein cows were divided into four groups as follows: Luteal phase, a DF was aspirated 7 days after GnRH injection; Pre-LH surge, a DF was aspirated 42 h after PGF2α injection during the mid luteal phase; Post-LH surge, a DF was aspirated 24 h after GnRH injection following PGF2α; and Intact follicle, ovulation was induced by GnRH injection after PGF2α.... |
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Palavras-chave: Corpus luteum formation; Cow; Follicle aspiration; LH surge. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2982 |
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| MIKI, Wataru; ONIYAMA, Hiroyuki; TAKEDA, Naomasa; KIMURA, Yuki; HANEDA, Shingo; MATSUI, Motozumi; TAYA, Kazuyoshi; NAMBO, Yasuo. |
| We observed structural changes in the follicles and uterus of heavy draft mares during estrus and examined the effect of a single injection of the gonadotropin-releasing hormone analog buserelin on ovulation and endocrine profiles. Twenty-two heavy draft mares were divided into a buserelin-treated group (n=8) and a control group (n=14). Mares were given an intramuscular injection of 40 µg buserelin when they presented signs of estrus to a teaser stallion, had ≥45 mm diameter follicles, and presented decreased uterine edema compared with the previous examination. The follicles and uterus were monitored using transrectal ultrasound imaging and measurement of blood levels of luteinizing hormone (LH), follicle-stimulating hormone (FSH), progesterone, and... |
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Palavras-chave: GnRH analog buserelin; Heavy draft mares; Ovulation; Single injection; Uterine edema. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4397 |
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| HAYASHI, Ken-Go; BERISHA, Bajram; MATSUI, Motozumi; SCHAMS, Dieter; MIYAMOTO, Akio; 松井, 基純; 宮本, 明夫. |
| Recent findings indicate that the changing profile of angiopoietins (ANPT) and their receptor Tie2 are closely associated with development and regression of the vascular network in the cyclic ovary. We previously reported that mRNA expression for the ANPT-Tie system in theca interna changes during bovine follicular development and atresia, and both ANPTs affect steroidogenesis in the preovulatory follicle. The aim of this study was to investigate mRNA expression for ANPT1, ANPT-2 and Tie2 in granulosa cells (GC) during follicular development in the cow. Bovine follicles were classified according to the estradiol-17β (E2) concentration in follicular fluid (FF) as follows: (1) E2<0.5, (2) 0.5<E2<5, (3) 5<E2<20, (4) 20<E2<180 and (5)... |
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Palavras-chave: Follicular development; Angiopoietin-Tie system; Granulosa cell; Cow. |
| Ano: 2004 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2975 |
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| ACOSTA, Tomas J.; TETSUKA, Masa; MATSUI, Motozumi; SHIMIZU, Takashi; BERISHA, Bajram; SCHAMS, Dieter; MIYAMOTO, Akio; 松井, 基純; 清水, 隆; 宮本, 明夫. |
| The aim of the present in vivo study was to monitor real-time fluctuations of cortisol (Cr) in the wall of preovulatory follicles using a microdialysis system (MDS) implanted in the theca layer as well as changes in ovarian venous plasma (OVP) and jugular venous plasma (JVP). Seven cows were superovulated using FSH and prostaglandin F2α injections. Dialysis capillary membranes were surgically implanted into the theca layer of mature follicles and connected to a microdialysis system. Fractions of the perfusates were collected from Day -1 (Day 0=LH surge) to Day 3. No difference in the concentrations of Cr between JVP and OVP was detected throughout the experiment. Circulating concentrations of Cr ranged from 20 to 35 ng/ml 8 h after surgery in ovulatory and... |
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Palavras-chave: Bovine; Cortisol; Follicle; Ovary; Ovulation. |
| Ano: 2005 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2980 |
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| KAWASHIMA, Chiho; ITO, Nozomi; NAGASHIMA, Shuntarou; MATSUI, Motozumi; SAWADA, Kumiko; SCHWEIGERT, Florian J.; MIYAMOTO, Akio; KIDA, Katsuya; 川島, 千帆; 松井, 基純; 宮本, 明夫; 木田, 克弥. |
| The aim of the present study was to investigate nutritional and metabolic parameters during the dry and early postpartum periods of ovulatory and anovulatory cows, as well as their postpartum reproductive performance. Blood samples from 20 multiparous Holstein cows were collected once a week from the far-off dry period to 3 weeks postpartum. Early postpartum (0–3 weeks) ovulation was confirmed using plasma progesterone concentration profiles, and cows were considered ovulatory if they had resumed luteal activity by this point (n = 9), whereas cows that had not were considered anovulatory (n = 11). Data from the ovulatory and anovulatory cows were analyzed separately for the far-off dry period (7–4 weeks prepartum), the close-up dry period (3–1 weeks... |
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Palavras-chave: Dairy cow; First ovulation; Hepatic enzyme; Liver function; Reproductive performance. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4308 |
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| KAWASHIMA, Chiho; MATSUI, Motozumi; SHIMIZU, Takashi; KIDA, Katsuya; MIYAMOTO, Akio; 川島, 千帆; 松井, 基純; 清水, 隆; 木田, 克弥; 宮本, 明夫. |
| During recent decades, milk production per cow has increased drastically due to improved management, nutrition, and genetic selection; however, the reproductive performance of high-producing dairy cows has been declining. One of the factors responsible for this low reproductive performance is negative energy balance (NEB). NEB affects the onset of first ovulation in early postpartum cows. It is generally accepted that early first ovulation positively relates to the resumption of normal ovarian function, first service, and conception rate in dairy cows. Hence, delayed first ovulation has a negative impact on subsequent fertility. The metabolic condition of cows in NEB shifts to catabolic metabolism, which in turn causes increased plasma growth hormone and... |
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Palavras-chave: Β-Carotene; Dairy cow; First follicular wave postpartum; First ovulation; Metabolic hormone. |
| Ano: 2012 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4041 |
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| MIURA, Ryotaro; HANEDA, Shingo; MATSUI, Motozumi; 羽田, 真悟; 松井, 基純. |
| The objective of our study was to compare the characteristics of the corpus luteum (CL) formed after ovulation of the dominant follicle (DF) of the first follicular wave (W1) and those of the CL formed after ovulation of the DF of the second (induced) follicular wave (W2). Non-lactating Holstein cows were used for this study. In Experiment 1, cows were treated with PGF2α and GnRH on days 6 and 8 (day 0 = day of follicular wave emergence) for W1 (n = 6) and W2 (n = 6), respectively. Dominant follicles were aspirated on day 9 to quantify the amounts of mRNA (VEGF120, VEGF164, FGF-2, StAR, P450-scc and 3β-HSD) in granulosa cells (GC). In Experiment 2, the size and blood flow area of the CL formed after ovulation of the DF in W1 (W1CL; n = 6) and W2 (W2CL; n =... |
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Palavras-chave: Blood flow; Corpus luteum; Cow; Follicular wave; Gene expression. |
| Ano: 2015 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4147 |
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| YAMAKAWA, Kazuhiro; AOKI, Takahiro; MATSUI, Motozumi; YAMADA, Kazutaka; ISHII, Mitsuo; 滄木, 孝弘; 山田, 一孝; 石井, 三都夫. |
| To establish a method to predict postpartum diseases using prepartum back fat thickness (BFT), the peripartum BFTs of 54 healthy multiparous cows before calving, which were diagnosed with postpartum displacement of the abomasum (DA), clinical ketosis or subclinical ketosis were compared with those of healthy cows from 8 weeks before the expected calving date to 8 weeks after calving. The peripartum BFTs of the cows with DA or clinical ketosis were significantly higher than those of healthy cows. The peripartum BFTs of the cows with subclinical ketosis were not significantly higher than those of the healthy cows. |
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Palavras-chave: Back fat thickness (BFT); Clinical ketosis; Displacement of the abomasum (DA); Obese cow. |
| Ano: 2012 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3903 |
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| SHIRASUNA, Koumei; SASAHARA, Kiemi; MATSUI, Motozumi; SHIMIZU, Takashi; MIYAMOTO, Akio; 白砂, 孔明; 松井, 基純; 清水, 隆; 宮本, 明夫. |
| Administration of prostaglandin (PG) F2α in cattle during the mid-luteal phase (Days 8-12 of the estrous cycle) drastically reduces the plasma progesterone concentrations and the volume of the corpus luteum (CL). However, PGF2α does not induce luteolysis during the early luteal phase (up to Day 5 of the estrous cycle). To characterize the possible distinct difference in acute response to a luteolytic dose of PGF2α administration, we determined various mRNA expressions in the early and mid CL relating to angiogenesis, vasoactivation and PG-related factors at 30 min after PGF2α injection in cyclic cows. The experiments were conducted on Day 4 (early CL) and Days 10-12 (mid CL). Cows were either injected with 500 μg PGF2α analogue or saline as the control... |
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Palavras-chave: Corpus luteum; Cow; Luteolysis; Prostaglandin F2α. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2812 |
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| ISHII, Mitsuo; KOBAYASHI, Syuichi; ACOSTA, Tomas J.; MIKI, Wataru; YAMANOI, Takahiro; MATSUI, Motozumi; MIYAKE, Yoh-Ichi; MIYAMOTO, Akio; 石井, 三都夫; 松井, 基純; 三宅, 陽一; 宮本, 明夫. |
| The aim of this study was to clarify the relationship between circulating oxytocin (OT) and PGF2α metabolite (PGFM) in mares at the third stage of labor and placental expulsion time in order to investigate a cause of retained placenta of which the incidence increase in a heavy draft mare. Blood was sampled every 5 min from foaling to expulsion of the placenta in 18 heavy draft mares to evaluate circulating OT and PGFM. The relationships between OT and PGFM concentration and recorded placental expulsion times were investigated. The results were as follows (1) The highest level of OT concentration was observed close to foaling in 15 mares. (2) The OT concentrations close to foaling were variable with a large difference from the lowest concentration, 22.1... |
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Palavras-chave: Heavy draft mare; Oxytocin; 15-keto-dihydro prostaglandin F2α (PGFM); Placental expulsion. |
| Ano: 2008 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3089 |
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| KAWASHIMA, Chiho; MUNAKATA, Megumi; SHIMIZU, Takashi; MIYAMOTO, Akio; KIDA, Katsuya; MATSUI, Motozumi; 川島, 千帆; 清水, 隆; 宮本, 明夫; 木田, 克弥; 松井, 基純. |
| This study aimed to investigate the effects of insulin resistance (IR) during the close-up dry period on the metabolic status and performance of dairy cows as well as to determine the effects on body weight (BW) and metabolic status of their calves. An insulin tolerance test (ITT) was conducted by administering 0.05 IU/kg BW of insulin to 34 multiparous Holstein cows at 3 weeks prepartum. Blood samples were collected at 0, 30, 45 and 60 min after insulin injection, and cows were divided into two groups based on the time required for glucose to reach the minimum levels [non-IR (NIR), 45 min (n=28); and IR, 60 min (n=6)]. Blood or milk sampling and body condition score (BCS) estimation were performed twice weekly during the experimental period. Blood samples... |
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Palavras-chave: Dairy cow; Dry period; Insulin tolerance test; Metabolic status; Postpartum performance. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4363 |
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| KAWASHIMA, Chiho; KANEKO, Etsushi; AMAYA MONTOYA, Carlos; MATSUI, Motozumi; YAMAGISHI, Norio; MATSUNAGA, Nobuyoshi; ISHII, Mitsuo; KIDA, Katsuya; MIYAKE, Yoichi; MIYAMOTO, Akio; 川島, 千帆; 松井, 基純; 石井, 三都夫; 木田, 克弥; 三宅, 陽一; 宮本, 明夫. |
| The aim of this study was to investigate the relationship between the first ovulation within 3 weeks postpartum and subsequent ovarian cycles and fertility in high producing dairy cattle in Hokkaido, Japan. In Experiment 1, 110 cows (44 primiparous and 66 multiparous) were used to determine the effects of the first ovulation within 3 weeks postpartum on subsequent ovarian cycles. Milk samples were collected twice weekly from 7 to 100 days postpartum. The first ovulation was identified by an increase in milk progesterone (P4) to more than 1 ng/ml within 3 weeks postpartum. The numbers of cows showing ovulation and anovulation within 3 weeks postpartum were 31 (70.5%) and 13 (29.5%) in the primiparous cows and 35 (53.0%) and 31 (47.0%) in the multiparous... |
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Palavras-chave: Dairy cow; First ovulation; Postpartum; Resumption of ovarian cycle. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/916 |
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| SHIRASUNA, Koumei; KAWASHIMA, Chiho; MURAYAMA, Chiaki; AOKI, Yuka; MASUDA, Yutaka; KIDA, Katsuya; MATSUI, Motozumi; SHIMIZU, Takashi; MIYAMOTO, Akio; 白砂, 孔明; 川島, 千帆; 増田, 豊; 木田, 克弥; 松井, 基純; 清水, 隆; 宮本, 明夫. |
| The decrease in fertility and conception rates of high-producing dairy cows is one of the major negative impacts for today's producers. The recovery of ovarian activity postpartum is affected by the status of immunity, metabolism and reproduction and plays a critical role in subsequent fertility after parturition in the cow. In the present study we investigated the relationships between polymorphisms in genes relating to the above functions and the first postpartum ovulation as a marker of the recovery of ovarian function in the cow. In immune function related-factors, the occurrence of first postpartum ovulation within 3 weeks in the C/C genotypes of tumor necrosis factor α (TNFα) exon (55.4%) and the A/G genotypes of TNFα promoter (55.4%) was... |
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Palavras-chave: Cow; Ovulation; Polymorphism; Tumor necrosis factor α (TNFα). |
| Ano: 2011 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2990 |
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| Registros recuperados: 24 | |
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